Translational_Unit

Part:BBa_K3803012:Design

Designed by: Shiyu Yu   Group: iGEM21_Jiangnan_China   (2021-09-02)


MT-Ox translational unit


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 166
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 166
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 166
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 166
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The codon of MT-Ox from Danio rerio had been optimized for better expression in S. cerevisiae.


Source

The promoter and terminator is from S. cerevisiae, and the coding gene of MT-Ox is from Danio rerio.

References